FastQCFastQC Report
Wed 11 May 2022
EGAF00005312781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30957047
Sequences flagged as poor quality0
Sequence length120
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2843470.9185210721164716No Hit
GCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGG1567120.5062239948144925No Hit
GCTCCGATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGG1086700.35103477408552564No Hit
GCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCC934970.3020217012300947No Hit
GCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAA929620.3002935002166066No Hit
GCTCCCATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGG890320.2875984909025722No Hit
ATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTG849660.27446416320006234No Hit
GCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGC823170.2659071454715949No Hit
CGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG812100.26233122300069517No Hit
GCTCCTATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGG727060.23486090259190417No Hit
AGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG639600.20660885387420838No Hit
GCCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG596930.19282523943578986No Hit
TGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG588700.1901667171290595No Hit
GCCCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGG570370.184245609731445No Hit
TGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTC552360.17842787136641294No Hit
GGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG531370.17164750888545668No Hit
TCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACA523030.1689534534737761No Hit
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC485000.1566686900078034No Hit
GCTTCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGG460950.14889986115277726No Hit
GCACCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGG449410.145172115415272No Hit
GTTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGG432250.13962895104303716No Hit
GTAACTTTTTTGTGTATGTACTTAGCTGTACTATAAGTAGTTGGTTTGTA419000.13534882703766932No Hit
GGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAA418200.13509040445621315No Hit
GCTCTAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGG405650.13103640020961949No Hit
ACTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGG383750.12396208204225681No Hit
GCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGT372060.12018588207072853No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC362360.11705250827057244No Hit
TCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTC325840.10525551742709825No Hit
GCCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA319820.10331088750164057No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)