FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005312784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16900711
Sequences flagged as poor quality0
Sequence length30-75
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCGT13700.059.94802569
TTAGGCG17250.048.31579268
AGATTAG21900.038.89032465
AGTAGAT21650.037.93370462
CTGAAGG395550.037.291437
CGCGATA174150.037.101558
CCGAGAT229650.037.0990267
TCGCGAT175550.037.0444227
TGATGCC299650.037.0435647
CAGCTAT359000.036.98289
GCGATAT174750.036.952819
CGAGATT231000.036.9480558
AGCGAGT261500.036.942388
ACCCTAC243250.036.8958557
GATACCT212100.036.892019
AGCTATT356100.036.8880310
TCCAGCT385900.036.866937
TAGTTCC284200.036.8594868
GAAGGAT399850.036.8431059
CCCTACA242750.036.814858
Produced by FastQC (version 0.11.5)