FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005312786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11121477
Sequences flagged as poor quality0
Sequence length75
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCTC162100.067.271568
GCAAACT420950.066.930199
GCGAACT146350.066.9210368
TACGCCT167000.066.640747
GATCCTA141650.066.48698
GGAACGT118850.066.295949
TCGGATT91900.066.1800110
ATCCTAT143050.066.149719
CGAACTT148550.065.9763959
AACGAGT146600.065.936239
CTCGGAT92250.065.891529
GTATGCA164850.065.8775947
AGTTGCG115800.065.8375558
AGCGAAC149300.065.807017
ATACACG89600.065.569017
TCGTCCT46250.065.564557
CGCCTCT165450.065.534149
TATGCAC163800.065.520338
TATCTCG49550.065.4449547
GATCGAA71600.065.382097
Produced by FastQC (version 0.11.5)