FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005312790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15915204
Sequences flagged as poor quality0
Sequence length30-75
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGT191050.060.0451749
CCGAGTA233700.060.0387847
ACGATCC131300.059.9327857
CGGAAGT183950.059.8938337
GAGTACT232450.059.8222059
CGAGTAC233150.059.669668
AGTACTT233800.059.55698810
CTCACAT159950.059.3815849
TCGTACT109050.059.3487369
ACGCCTC81100.059.2919468
CGCTATA193650.059.2878957
TATCTCG57500.059.1659057
TTCGTAC109850.059.1448448
ATCCACG97350.059.0806058
ACTAGCG59950.059.047137
TCACGTG100650.058.9487238
TAGGTCA95700.058.9210057
GTCACTT169300.058.91441310
CGTACTT110000.058.80751410
TACGCCT83300.058.7791827
Produced by FastQC (version 0.11.5)