FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005312791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15915204
Sequences flagged as poor quality0
Sequence length75-120
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGCG51650.0116.98916114
AGCGCCC27450.088.635254114
CTCTCGG73050.087.664116112-113
CGAACGC30450.071.55723690-91
TACGAAC31900.070.8141888-89
AACGCAC32700.068.2710492-93
GATAGCG30650.063.2664346
TTAAAAC216150.061.105343114
CCTCTCG75250.060.94428110-111
CAGTCGC39050.060.26041102-103
CGATAGC26900.059.4875955
TTAACAG86700.059.209255114
GCCTCTC111000.058.29989110-111
CCGATAG23150.057.9000854
TTGCCTC111850.057.379322108-109
ACGAACG29450.056.73851490-91
TATAGCG16700.056.3663756
GGCGAGC120950.056.12558782-83
AGTCGCA33150.056.06428104-105
GTCGCAT42500.055.999752104-105
Produced by FastQC (version 0.11.5)