FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005312792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15436374
Sequences flagged as poor quality0
Sequence length30-75
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCGT11600.049.19732369
GGTGACT374750.037.7107937
GTGACTA374300.037.5973558
TGACTAT376450.037.4181869
GACTATT377850.037.25379610
CATGAGG269500.037.1397638
TCGACTC80350.037.0818337
AGTGGGA343200.037.0463457
GTGGGAT343300.037.030088
TCATGAG274350.036.97267
CGGTGAC106200.036.9125756
TTAGGCG15300.036.90730368
TGGTAGC190200.036.7976387
CGACGTT118450.036.75114410
GACGTTT173800.036.7367511
ATGCGAC119100.036.7281237
TACGTGT114500.036.6788649
TTCGCCT169900.036.6322069
ATGAGGT273750.036.619119
GTACGTG114950.036.618858
Produced by FastQC (version 0.11.5)