FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005312793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005312793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15436374
Sequences flagged as poor quality0
Sequence length75-120
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG857740.5556615821824478No Hit
GCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGG176160.11412006472504488No Hit
GTAACTTTTTTGTGTATGTACTTAGCTGTACTATAAGTAGTTGGTTTGTA168790.10934562741224073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGC35750.081.056215
GATAGCG42700.077.7760246
CCGATAG32150.077.589534
TATAGCG22750.065.439146
CTCGGCG193600.060.454308114
GCTCCGA26350.055.937791
CATAGCG32300.051.1786
AGCGCCC38150.048.571533114
CCCCGTA43700.046.257842
CGATTGG19550.045.5874825
ATAGCGT392950.042.6523447
CCCGTAC34700.042.615793
TAGCGTA404000.042.0298048
AGCGTAT412400.041.2224169
CTCTCGG290400.040.544983112-113
CGTACTA30050.040.0968445
CGAACGC58150.038.53479490-91
TCCGATA48350.038.102093
CTAGCGT11550.036.6417585
TACGAAC63200.036.5551188-89
Produced by FastQC (version 0.11.5)