FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9301542
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGA5350.024.85897334-35
CGCGAAA5150.024.43789736-37
CCGCGAA5900.022.54160934-35
CGTTGGA37100.017.8858531
TATGCCG7650.017.3829348-49
CTCCGCG8000.017.2192332-33
ACGGGAT25950.015.9216219
CGTCCGA1900.003761669414.9969536
AACGGGA35800.014.9899718
AAACGGG43500.014.8475587
TCTCGTA8700.014.73917742-43
CGTATGC9650.014.27232546-47
GAAACGG46950.013.9588256
GGAAACG46500.013.58326155
TCGTATG10700.013.31555344-45
ATGCCGT10800.013.19240248-49
CTCGTAT9800.013.08456944-45
TGCCGTC11450.012.85813350-51
GCGAAAT10050.012.75918536-37
ACTCCGC11100.012.41025732-33
Produced by FastQC (version 0.11.5)