FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4616965
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5350.027.96235848-49
GATCGGA11500.026.9815981
CACGACG1457.561048E-726.3374771
ACGACGC1501.0495332E-625.3656772
ATCGGAA12600.024.9127182
TCGTATC5700.024.5799338-39
CGTATGC6600.023.02624146-47
TCTCGTA6650.022.49676342-43
CTCGTAT6800.022.34948344-45
TCGGAAG14300.021.9263343
CGACGCT1805.167676E-621.1142483
ATGCCGT7550.020.44341348-49
GTCACGA8050.019.76659228-29
TATCTCG7600.019.37231640-41
TCGTATG8350.019.33832644-45
CGAATTC8450.019.1124732-33
CGTTGGA19300.018.7978611
CGGAAGA16500.018.7114564
GCCGTCT8000.018.69962550-51
ATTCGTA8550.018.05315436-37
Produced by FastQC (version 0.11.5)