FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5169403
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT60930.1178666085813004TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG10350.029.5970948-49
GATCGGA20900.029.2157731
TCTCGTA10300.029.0502442-43
ATCGGAA21400.028.891972
CGTATGC10550.028.81091946-47
TCGGAAG22050.027.7925473
CTCGTAT10900.027.45021844-45
GTCACGA11950.026.832328-29
TCGTATG12100.026.69041344-45
CGGAAGA23400.025.9823464
TGCCGTC11700.025.97882750-51
GCCGTCT12750.025.1431150-51
ATGCCGT12750.024.95710848-49
ATCTCGT12100.023.35490242-43
ACGAGAT13250.022.94489932-33
CGTCTTC14800.021.50041852-53
AGTCACG14300.021.42622828-29
CATCTCG14150.020.64283440-41
CACGAGA15650.020.4885630-31
CGAGATT16050.020.27391432-33
Produced by FastQC (version 0.11.5)