FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5169403
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT52500.10155911620742279Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA7750.039.11536858-59
TCGCCGT8400.036.92175756-57
GTCGCCG8450.035.59942656-57
GAGCGTC17300.034.5077369
GCCGTAT9750.032.2875458-59
AGAGCGT18800.031.2570868
CCGTATC10500.029.09210660-61
GGTCGCC11000.028.61988354-55
GATCGGA21050.028.5819471
TGGTCGC10600.028.37988754-55
CGTATCA10900.028.23843260-61
AAGAGCG21100.028.0712177
GTGGTCG11200.027.6934652-53
ATCGGAA22500.026.3268552
TCGGAAG23050.025.897263
CGGAAGA24600.024.6446724
TCGGTGG13200.023.6738150-51
GCGTCGT13050.023.40891310-11
CTCGGTG14250.023.07521648-49
TCTCGGT13400.022.97262648-49
Produced by FastQC (version 0.11.5)