FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7103405
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA7000.034.30889558-59
GTCGCCG8200.029.8567856-57
TCGCCGT8200.027.86632756-57
GAGCGTC17600.027.5553519
TGGTCGC9500.025.77184354-55
GCCGTAT9500.024.05304158-59
AGAGCGT21450.023.4779628
GGTCGCC10350.023.20473554-55
CCGTATC11050.022.57866360-61
GTGGTCG11050.021.31406852-53
GATCGGA23550.021.122091
CGTATCA11050.021.10155560-61
ACGACGC2501.17634045E-820.5174185
TCGGAAG24700.020.3897613
ATCGGAA24600.020.095362
CGGAAGA26200.019.2224064
AAGAGCG26400.019.076387
GCGTCGT13300.018.23211310-11
TCGGTGG14050.017.7587250-51
CGTCGTG13800.017.74067312-13
Produced by FastQC (version 0.11.5)