FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4910535
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT3200.027.4559736-37
TCGCGCA3350.026.22659936-37
TCTCGCG3450.026.15668734-35
TATGCCG4050.021.6939448-49
CTCGCGC4700.020.71591834-35
CGTATGC4700.019.70418246-47
GCGCATC4400.019.42909638-39
TCTCGTA4950.018.70959542-43
CTCGTAT4750.018.49712444-45
CGTTGGA18350.017.6965711
ATGCCGT6050.015.6998848-49
AAACGGG22050.015.291637
ACGGGAT13150.015.1679739
AACGGGA18000.015.0385648
ACTCTCG6100.014.79496632-33
TCGTATG6650.014.64058144-45
GAAACGG22250.014.5138596
TGCCGTC6600.014.0319150-51
CGCATCT6550.013.77669538-39
GCCGTCT7050.012.79942950-51
Produced by FastQC (version 0.11.5)