FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5445872
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA3450.035.146758-59
TCGCCGT4200.029.42503256-57
GTCGCCG4500.026.4270156-57
GAGCGTC10250.023.2045239
GCCGTAT5200.022.87005258-59
GATCGGA10600.022.7982061
GGTCGCC5750.021.899254-55
ATCGGAA11850.021.646052
TGGTCGC5700.021.27310454-55
ACGACGC2206.8568625E-821.1988182
GCGTCGT6050.020.4273910-11
AGAGCGT12300.019.7155388
CACGACG2201.3784047E-619.384571
CGTCGTG6250.019.02770812-13
GTGGTCG6950.018.7907252-53
CGACGCT2553.250443E-718.2883
AGCGTCG6400.018.21723210-11
TCGGAAG13850.018.1823653
CGTTGGA22350.017.384921
CGGAAGA15450.016.9030844
Produced by FastQC (version 0.11.5)