FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6454174
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT78480.12159573014300512TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG14200.030.9369948-49
TCGTATG15450.027.8189944-45
CTCGTAT15750.027.43987544-45
CGTATGC16100.026.84314746-47
TCTCGTA14750.026.40279242-43
CGATAGA16500.026.1928136-37
AGCGATA16850.025.51137734-35
GCGATAG17450.025.45079634-35
ATGCCGT16900.025.43235248-49
ATCTCGT17000.023.04798942-43
TGCCGTC18850.022.54929450-51
GCCGTCT19550.021.25605250-51
CAGCGAT22300.019.70375432-33
TCGGAAG44800.019.6171633
ATCGGAA44300.019.5386712
GATCGGA44800.019.1812081
CGGAAGA46100.018.8561154
CCGTCTT21500.018.77652452-53
CGCCGTA1801.2365312E-418.4681438
GTATGCC24100.017.8340346-47
Produced by FastQC (version 0.11.5)