FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4538816
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT66510.14653601291614377Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA15350.053.741611
GAGCGTC14350.050.9751439
ATCGGAA16700.047.7776762
TCGGAAG17500.045.5940553
AGAGCGT16450.045.0436948
AAGAGCG18150.042.655667
CGCCGTA9050.040.9293858-59
CGGAAGA19550.040.0842864
TCGCCGT9150.039.96306656-57
GTCGCCG9650.038.3845556-57
GCCGTAT9800.037.5547458-59
TCGGACG1152.5083864E-937.1690673
TGGTCGC10600.035.61584554-55
GCGTCGT10600.034.28280310-11
CGTATCA11200.033.7087860-61
GGTCGCC11700.032.6732254-55
CGTCGTG11000.032.60556412-13
GTGGTCG11950.032.38778352-53
CCGTATC11200.032.0127460-61
AGCGTCG11550.031.66864610-11
Produced by FastQC (version 0.11.5)