FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5125316
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT58070.11330033114055797Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA9300.040.62337558-59
GTCGCCG9650.038.42479756-57
GAGCGTC20450.038.0878529
TCGCCGT10150.037.22123756-57
AGAGCGT21250.037.0922058
GGTCGCC11100.034.6660954-55
GCCGTAT11600.034.1770758-59
TGGTCGC11200.033.31546454-55
GTACGTC11650.033.03062430-31
GTGGTCG11800.032.61185552-53
GATCGGA24300.031.9713271
ATCGGAA24750.031.6621042
GTGTACG12700.031.21761528-29
AAGAGCG25400.030.8485417
TACGTCC12200.030.77674532-33
TCGGAAG25700.030.669623
CGTATCA13100.029.72916660-61
CGGAAGA26600.029.2812544
CCGTATC12950.029.17311560-61
TGTACGT14150.026.7003730-31
Produced by FastQC (version 0.11.5)