FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3533922
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA2450.035.8593258-59
GCCGTAT3200.026.71277458-59
TCGCCGT3450.025.46495656-57
GTCGCCG3800.023.74434956-57
GAGCGTC8100.022.2836959
GATCGGA8700.021.6277661
TGGTCGC4150.021.16831254-55
CCGTATC4050.021.10668860-61
ATCGGAA9450.019.5987872
GGTCGCC4800.018.79641354-55
CGTATCA4950.018.708260-61
GTGGTCG4850.018.60303152-53
GGTACGA1300.006915223318.5563681
TCGGAAG10250.018.5329633
AGAGCGT10150.018.2502468
CGGCATA2453.7681002E-617.4480388
AGCGTCG5300.017.02810710-11
GCGTCGT5150.016.6017510-11
TACGGCA2304.281084E-516.5189766
CGGAAGA11450.016.1763364
Produced by FastQC (version 0.11.5)