FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5451246
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA4750.036.8167258-59
TCGCCGT5050.034.63006256-57
GAGCGTC11650.031.6226759
GTCGCCG5850.030.6915156-57
GCCGTAT6350.029.00886758-59
GATCGGA14150.027.1224371
TCGGAAG14900.025.6633343
ATCGGAA15350.025.5244262
GGTCGCC7250.025.0868454-55
AGAGCGT14700.025.0615088
CCGTATC7450.024.41213660-61
TGGTCGC7500.023.62880154-55
GTGGTCG8200.023.0342752-53
CGTCGTG7950.022.87657212-13
CGTATCA8200.022.17931760-61
AAGAGCG17500.021.5846217
CGGAAGA17300.021.5640034
AGCGTCG8700.020.90464410-11
GCGTCGT8650.020.75592410-11
GTCGTGT10000.017.72054512-13
Produced by FastQC (version 0.11.5)