FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5560759
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA25550.017.9397221
AAACGGG28800.016.6550667
ACGGGAT18000.016.3582429
AACGGGA24050.015.9951148
GATCGGA11700.015.507191
TCTCGTA5750.015.28061642-43
TATGCCG5450.015.25016748-49
GGAAACG30350.015.0220775
GAAACGG31400.014.3684996
ATCGGAA13100.014.1588552
TCGGAAG13200.014.0356683
CTCGTAT5900.013.68428744-45
AACGGGT5402.0991138E-913.192138
ATGCCGT6900.013.07788748-49
TCGTATG6950.012.98356844-45
CGTATGC6550.012.68884246-47
CGGAAGA15350.011.7587994
TGCCGTC7700.010.79394950-51
ATCTCGT7800.010.35121442-43
CCGGATT3800.002926523610.012472
Produced by FastQC (version 0.11.5)