FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3424856
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4750.029.99741248-49
CGTATGC5400.026.3871946-47
CGATAGA5650.024.79820336-37
AGCGATA6100.023.74742934-35
TCGTATG6550.023.56718444-45
CTCGTAT6100.023.3591544-45
TCTCGTA5850.023.13919842-43
ATGCCGT6350.022.43900948-49
GCGATAG6500.022.2860534-35
TGCCGTC6200.021.06765450-51
GCCGTCT6200.020.68460550-51
ATCTCGT7300.018.54305542-43
CAGCGAT8600.017.67224932-33
CGTCTTC6500.016.80734652-53
CGGAAGA18150.016.4860614
ATCGGAA18700.016.0238692
TCGGAAG18750.015.9582743
ACAGCGA9150.015.83138932-33
GATCGGA18750.015.73644351
AGGTTCG2450.001289140913.5701594
Produced by FastQC (version 0.11.5)