FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4291415
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA3400.037.0123558-59
TCGCCGT4000.030.86744356-57
GTCGCCG4350.030.5672356-57
GAGCGTC11000.024.181119
ATACGGC2853.6379788E-1223.3285735
GCCGTAT5300.023.29577458-59
AGAGCGT12450.022.8895658
GATCGGA12250.022.8486561
TGGTCGC6450.021.71948854-55
GGTCGCC6250.021.65469754-55
CGTATCA6150.020.84806860-61
ATCGGAA13600.020.2541642
GAGCCGT7450.020.08283836-37
TCGGAAG14000.020.0137753
CCGTATC6450.019.51027360-61
GCGTCGT6700.019.498210-11
GTGGTCG7350.019.38334852-53
CCGTGTA7700.019.1199238-39
CGTCGTG6850.019.07256312-13
CGGAAGA15450.018.750224
Produced by FastQC (version 0.11.5)