FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3830124
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA6550.035.6020558-59
TCGCCGT6850.033.36132456-57
GTCGCCG7400.032.7724956-57
GCCGTAT7500.031.09245758-59
CCGTGTA8900.029.44674538-39
TGGTCGC8350.029.3231554-55
ACGACGC1602.3192115E-929.1516512
CCGTATC8000.028.85787660-61
GGTCGCC8650.028.03658354-55
TACGGCG1005.6160647E-527.9819366
CGTATCA8650.027.22854660-61
GAGCGTC18550.026.146649
GTGGTCG9400.026.04871252-53
AGAGCGT19500.024.6336698
GAGCCGT11650.024.39030636-37
AAGAGCG22200.022.2679047
TCTCGGT11500.021.69868548-49
CACGACG2205.7854777E-821.5418321
GCCGTGT12350.021.47038538-39
GATCGGA24200.021.1501621
Produced by FastQC (version 0.11.5)