FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4138117
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT59510.14380937030054974Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC15400.051.1783149
GATCGGA16500.048.5305861
ATCGGAA16800.048.0215072
AGAGCGT17000.046.6352968
TCGGAAG18100.045.3453833
CGCCGTA9150.042.55763658-59
CGGAAGA19150.042.372054
AAGAGCG20300.039.7433787
TCGCCGT9850.038.82282356-57
GTCGCCG10300.038.03220756-57
CCGTGTA11150.036.54664638-39
GCCGTAT10700.036.17482458-59
CGGCGTA651.4804196E-435.8733028
TGGTCGC11000.035.18823654-55
GGTCGCC11300.035.07943354-55
GTGGTCG11650.034.02636752-53
CCGTATC11900.032.91903360-61
GCGTCGT12300.031.65911310-11
GAGCCGT14200.031.6201236-37
CGGCATA2400.031.09028
Produced by FastQC (version 0.11.5)