FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6722674
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA4350.031.62745958-59
TCGCCGT5250.025.31718656-57
GTCGCCG5800.023.31846256-57
GAGCGTC12600.022.2084739
GCCGTAT6550.021.71651358-59
GATCGGA13100.021.337641
ATCGGAA15050.019.2146282
AGAGCGT14600.019.1657898
TGGTCGC7250.018.97689854-55
GGTCGCC7650.017.98464254-55
TCGGAAG16100.017.9592273
CCGTATC8300.017.70001860-61
GCGTCGT8150.017.45334210-11
GTGGTCG8350.016.75715352-53
CGTTGGA24200.016.2490651
CGTCGTG8500.015.91153912-13
AGCGTCG8950.015.89326710-11
CGGAAGA18750.015.1722654
AAGAGCG19400.015.1449247
CGTATCA9100.015.118957560-61
Produced by FastQC (version 0.11.5)