FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4226830
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7150.033.21322348-49
GATCGGA15650.030.4089811
CGTATGC8050.030.0892346-47
ATCGGAA16200.029.9484882
TCGGAAG16300.029.4279673
CTCGTAT8300.029.18361944-45
TCTCGTA7850.028.13392642-43
TCGTATG8500.027.93818344-45
TATCTCG8200.026.9327740-41
ATGCCGT9200.026.328748-49
CGGAAGA18350.025.8821724
TGCCGTC9200.023.23230650-51
ATCTCGT10250.022.473242-43
GCCGTCT9550.021.1374850-51
CGTCTGA12700.019.25906216-17
ACACGTC13450.017.83223712-13
CCGTCTT10800.017.5915452-53
ACGTCTG14400.017.15022914-15
CGTCTTC11250.016.25458152-53
GTATGCC15400.015.88266146-47
Produced by FastQC (version 0.11.5)