FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2863428
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA6500.038.8167151
TCGGAAG7200.035.618643
ATCGGAA7600.033.79652
TATGCCG4000.029.68479348-49
CGGAAGA8800.029.1425254
CTCGTAT4550.025.5759344-45
CGCTCAT4950.025.42608634-35
TCTCGTA4400.025.36789142-43
TCGTATG4750.024.4990544-45
CGTATGC5050.023.51373546-47
ATGCCGT5000.023.27287748-49
CACCGCT5650.022.70021630-31
TATCTCG5250.022.61639240-41
ACCGCTC5750.021.8881632-33
GCCGTCT4700.021.72788250-51
TGCCGTC5050.021.16254850-51
ATCTCGT5300.021.06013742-43
ACACGTC6700.019.13954512-13
GTCACCG6650.018.92998528-29
CCGCTCA6850.018.7199432-33
Produced by FastQC (version 0.11.5)