FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5876756
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA14750.033.2390061
TATGCCG7750.031.25521348-49
ATCGGAA16650.030.2837332
TCGGAAG16950.029.9816233
AATGCGC8700.028.11441234-35
CGGAAGA19100.027.8502714
TAATGCG9000.027.44112634-35
GCGCATC8650.027.17879138-39
CTCGTAT9000.027.17842544-45
ATGCGCA9650.025.83884236-37
TCTCGTA9100.025.0526642-43
CGTATGC9900.024.70765946-47
TGCGCAT9850.024.34984436-37
ATGCCGT10150.023.39688148-49
TCGTATG10600.023.0760244-45
TGCCGTC10500.021.7121250-51
CGCATCT11350.020.71335238-39
GCCGTCT11150.020.2334150-51
ACACGTC13750.018.82507512-13
ATCTCGT13200.017.63096842-43
Produced by FastQC (version 0.11.5)