FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3956803
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5400.027.70557248-49
GATCGGA10950.027.3866121
TTACTCG5850.025.97966234-35
TCTCGTA5800.025.38524242-43
CTCGTAT5850.025.16875344-45
ATCGGAA12050.024.8705232
TCGGAAG12600.024.1230563
CGGAAGA13350.023.4796184
TACTCGA6500.023.01635636-37
CGTATGC6500.022.65187546-47
TCGTATG6550.022.47895844-45
ATGCCGT7450.021.67569448-49
ATCTCGT7400.020.21745342-43
TGCCGTC7050.020.21100850-51
GCCGTCT7100.018.39628850-51
ACTCGAT9250.016.4303836-37
ACACGTC9700.016.40217612-13
CGTCTGA9900.015.35161716-17
CGTCTTC8000.014.84245852-53
ACGGGAT10750.014.5834469
Produced by FastQC (version 0.11.5)