FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3956803
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA3500.040.0260158-59
TCGCCGT4300.031.47393256-57
GTCGCCG4350.031.11216256-57
GAGCGTC9250.030.8103529
TGGTCGC5000.028.49183354-55
AGAGCGT10500.027.1403948
GATCGGA10500.027.1151391
GCCGTAT5400.026.822258-59
GGTCGCC5300.026.431154-55
AAGAGCG11900.025.542617
ATCGGAA10850.025.3852312
TCGGAAG12050.023.6469143
GCGTCGT6450.022.8308510-11
CCGTATC6350.022.43564860-61
GTGGTCG6300.022.2363952-53
CGTATCA6450.022.08780960-61
CGTCGTG6550.021.75787712-13
TCTTCGC6450.021.72846632-33
AGCGTCG6800.021.65573110-11
CGGAAGA13100.021.3895664
Produced by FastQC (version 0.11.5)