FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7046731
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT100230.14223616596120953TruSeq Adapter, Index 8 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA27000.038.4321981
TCGCGCA13450.038.13709636-37
CGCGCAT12200.037.95691736-37
ATCGGAA28200.036.9421962
TCTCGCG14100.036.54742434-35
CGGAAGA29450.035.4799964
TATGCCG14500.035.37657548-49
TCGGAAG29750.035.121713
CTCGCGC15700.033.27661534-35
CTCGTAT15500.032.94088444-45
TCGTATG16400.031.71237444-45
CGTATGC16400.031.56756846-47
TCTCGTA15050.031.08508342-43
GCGCATC14950.030.97487638-39
ATGCCGT16600.030.75816348-49
TGCCGTC16150.028.2334150-51
CGCATCT16500.028.06511538-39
ACTCTCG19200.026.96291232-33
ATCTCGT17100.026.80300542-43
GCCGTCT17350.025.59627550-51
Produced by FastQC (version 0.11.5)