FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7046731
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCT85130.12080779016539725Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC19750.045.69469
GATCGGA24050.041.3261451
CGCCGTA11450.040.6463858-59
GTCGCCG12700.037.39296756-57
TCGCCGT11950.037.1567256-57
AGAGCGT24800.036.771348
ATCGGAA26600.035.8872682
TCGGAAG26750.035.5089953
CGGAAGA28200.033.6834144
GCCGTAT14050.033.4626458-59
AAGAGCG29250.032.3116657
TGGTCGC15050.031.86826954-55
CCGTATC15400.031.1463160-61
GGTCGCC15250.030.9832454-55
CAGTACG15750.030.76148234-35
CGTATCA15550.030.54045960-61
TCGGACG2051.8189894E-1230.1176913
GTGGTCG15850.030.10997652-53
AGTACGT16450.029.16290536-37
AGCGTCG15700.028.89301710-11
Produced by FastQC (version 0.11.5)