FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3875392
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4750.030.996448-49
ATCGGAA12100.027.1240542
CTCGTAT5900.026.16339944-45
GATCGGA12250.025.6413041
CGTATGC5850.025.5747846-47
CGGAAGA12600.025.2554954
TCGGAAG12600.025.2545173
TTACTCG6500.023.7480134-35
TACTCGA6500.023.01730336-37
ATGCCGT6450.022.82680948-49
TCTCGTA6150.021.62448342-43
TCGTATG7050.020.5481944-45
GCCGTCT6600.019.79000950-51
TGCCGTC6750.019.70205150-51
ATCTCGT7350.018.74016842-43
CCGTCTT7350.016.80131352-53
CGTCTGA9200.016.7776216-17
CGTCTTC7650.015.83200652-53
ACTCGAT8850.015.83200636-37
ACACGTC10200.015.13324312-13
Produced by FastQC (version 0.11.5)