FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3875392
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA3500.037.31527358-59
TACGACG550.002582301634.5402036
TCGCCGT4400.030.75879756-57
GTCGCCG4450.030.4131956-57
GAGCGTC8500.029.6181139
ATCGGAA10800.026.8218572
GCCGTAT5050.026.8025158-59
GATCGGA10650.026.7247811
CGTATCA5450.026.14230760-61
AGAGCGT10050.025.9917438
GGTCGCC5350.025.739154-55
TCGGAAG11350.025.5250823
TGGTCGC5350.024.85154554-55
CCGTATC5600.024.16996460-61
CGTCGTG5800.022.11456112-13
GCGTCGT5950.021.9551210-11
AAGAGCG11850.021.642567
GTGGTCG6200.021.44392452-53
CGGAAGA13100.021.0276094
AGCGTCG6350.020.19808210-11
Produced by FastQC (version 0.11.5)