FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4653899
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT5000.035.62047236-37
GATCGGA11650.034.7223851
ATCGGAA12000.033.29552
TCGCGCA5750.033.03928436-37
TCTCGCG5950.032.32782734-35
TATGCCG6300.030.90960148-49
CTCGCGC6400.030.7968734-35
TCGGAAG13500.029.5511673
CGGAAGA14050.029.0707264
GCGCATC6450.027.24445338-39
TCTCGTA7050.025.59944742-43
CGTATGC7950.025.38985646-47
ACGACGC950.001225874425.0043475
CTCGTAT7750.024.81942744-45
TCGTATG8600.023.74698344-45
ACTCTCG8250.023.60293632-33
ATGCCGT8800.022.12846648-49
ATCTCGT8550.020.55283442-43
CGCATCT8450.020.51503638-39
TGCCGTC8800.020.5094250-51
Produced by FastQC (version 0.11.5)