FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4653899
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA4650.041.36330458-59
GATCGGA11300.035.8662951
GTCGCCG5950.033.1237656-57
GAGCGTC11350.032.2218139
ATCGGAA12400.032.171462
TCGCCGT6050.030.6138356-57
AGAGCGT12250.029.8535378
GCCGTAT6550.029.36478858-59
TCGGAAG13650.028.1806833
CGGAAGA14100.027.6184024
GCGTCGT6950.026.65460410-11
CGTCGTG7300.025.70350612-13
GGTCGCC7550.025.47417854-55
GTGGTCG7450.025.1784152-53
AAGAGCG14750.025.113997
TGGTCGC7600.024.9941654-55
AGCGTCG7700.024.0583810-11
CCGTATC8750.023.06727860-61
CGTATCA8650.022.78491660-61
TCGTGTA9400.020.71925714-15
Produced by FastQC (version 0.11.5)