FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4978455
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT60990.12250788648285461TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA8300.034.6193636-37
TCCGCGA8750.033.11465534-35
CCGCGAA9400.032.08812734-35
CTCGTAT9300.030.89607444-45
TCTCGTA9300.029.87546542-43
CTCCGCG10450.028.86468332-33
TATGCCG10150.028.30914148-49
TCGTATG10950.027.97541244-45
CGTATGC10500.027.81754746-47
GATCGGA22850.026.3210891
ATGCCGT11450.025.71719648-49
GCCGTCT11500.025.60512550-51
GCGAAAT10800.025.06645236-37
ATCGGAA24950.024.4008772
ACTCCGC12050.024.24361832-33
TCGGAAG25100.024.0379983
TGCCGTC11650.023.84860650-51
ATCTCGT11350.023.43332742-43
CGGAAGA26050.022.9743824
CGTCTTC13850.020.23214152-53
Produced by FastQC (version 0.11.5)