FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5381351
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGA4500.034.8325834-35
CGCGAAA4400.034.00099636-37
CCGCGAA4900.031.98910334-35
TATGCCG6300.024.87764248-49
CTCCGCG6550.024.65687232-33
CTCGTAT6850.022.1866244-45
CGTATGC7250.021.61781346-47
TCTCGTA7150.020.59166342-43
ACTCCGC7800.020.40095932-33
TCGTATG8100.020.228644-45
GCGAAAT7300.019.84315736-37
GATCGGA16850.019.2641411
ATGCCGT8550.018.60863348-49
GCCGTCT8400.018.37518950-51
TCGGAAG17750.018.2011893
ATCGGAA18350.018.145132
TGCCGTC8750.017.91156850-51
CACTCCG9800.017.206930-31
CGTTGGA21250.017.072431
AACGGGA18950.016.5405658
Produced by FastQC (version 0.11.5)