FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5381351
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA4650.031.09065458-59
GTCGCCG5700.027.81834256-57
TCGCCGT5350.027.45895256-57
TGGTCGC6300.024.42893854-55
GCCGTAT6250.024.2507158-59
GAGCGTC12250.023.9841069
GGTCGCC6650.023.14320654-55
GTGGTCG7600.020.86530752-53
CCGTATC7200.020.07910260-61
CGTATCA7550.019.76596860-61
CGGCATA3555.456968E-1219.7050698
AGAGCGT15750.019.2463268
TCGGAAG17500.018.1209893
GATCGGA17000.018.1113641
ATCGGAA17800.017.5550542
GCGTCGT8750.017.05536510-11
CGTCGTG8850.016.862112-13
CGTTGGA19900.016.4241431
CGGAAGA18800.016.3718264
AAGAGCG19200.016.0308957
Produced by FastQC (version 0.11.5)