FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3502215
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4200.030.5327848-49
CTCGTAT4850.026.44283344-45
TCGTATC4750.025.99768838-39
CGTATGC4950.025.90789646-47
TATCTCG5050.025.39414240-41
TCTCGTA5150.024.44114342-43
GTCACGA5550.024.39285528-29
TCGTATG5500.023.74958444-45
GATCGGA12250.022.5318681
ATCGGAA12800.021.9245112
ATGCCGT5800.021.70049948-49
ATCTCGT6050.021.19782442-43
ATTCGTA5850.021.1089336-37
AATTCGT6450.020.98620434-35
CGAATTC6500.020.82432732-33
TCGGAAG13000.020.458553
GAATTCG6850.019.76073534-35
ACGAATT6800.019.55638532-33
CACGAAT7150.018.93431530-31
AGTCACG7550.018.24575428-29
Produced by FastQC (version 0.11.5)