FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3883318
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4400.028.60570148-49
TCGTATC4500.027.96893938-39
CTCGTAT5200.026.03160544-45
CGTATGC5350.023.97007446-47
TATCTCG5600.023.32285340-41
TCGTATG5700.022.49822844-45
TCTCGTA5600.022.47547542-43
ATTCGTA6650.020.35475336-37
GATCGGA13800.020.3493441
GTCACGA7000.019.34124828-29
TCGGAAG15050.019.2499853
ATCGGAA14600.019.2224062
CGTATCT6800.019.20730438-39
GAATTCG7650.018.93568634-35
CGGAAGA15150.018.4961784
CGAATTC8050.018.28966132-33
AATTCGT7550.018.24289134-35
ATGCCGT6700.018.07693548-49
TGCCGTC7100.017.72793450-51
GCCGTCT7150.017.60396250-51
Produced by FastQC (version 0.11.5)