FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3883318
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA3350.037.5665458-59
TCGCCGT4100.032.43204556-57
GCCGTAT4100.032.43204558-59
GTCGCCG4700.027.28136356-57
TGGTCGC4900.026.16682854-55
GAGCGTC10500.025.329849
CCGTATC5100.025.14229660-61
GGTCGCC5300.025.08797354-55
CGTATCA5600.023.745560-61
AGAGCGT11550.023.4377238
GTGGTCG5950.022.7466252-53
GTCTTCG6850.019.41400730-31
ATCGGAA14600.018.8660472
TCTTCGC7150.018.269332-33
GATCGGA14350.018.1545431
AAGAGCG15750.017.7895937
CTTCGCC7900.017.43677532-33
TCTCGGT7800.017.35404648-49
TCGGAAG16300.017.189113
TTCGCCT7700.016.96391534-35
Produced by FastQC (version 0.11.5)