FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5793750
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6700.023.74803248-49
CGTATGC7250.021.61936646-47
CTCGTAT7600.020.31160744-45
TCGTATG8050.019.76621244-45
TCTCGTA7550.019.50203342-43
ATGCCGT8900.018.67822848-49
ATCGGAA18850.017.9159742
GATCGGA18700.017.8177971
TCGGAAG19300.017.2255883
CGGAAGA21350.016.2392434
ATCTCGT9050.016.00723842-43
GCCGTCT8750.015.47041550-51
CGTTGGA19650.015.0185061
TGCCGTC10000.014.48655150-51
ACGGGAT15350.013.9268739
AACGGGA20650.013.5731758
AAACGGG24950.012.7571537
CGTCTGA12950.012.46963316-17
CGTCTTC10800.012.31395452-53
GTATGCC15450.011.98955746-47
Produced by FastQC (version 0.11.5)