FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5793750
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA4000.034.4289258-59
GTCGCCG5950.026.33786256-57
TCGCCGT5550.025.24134856-57
GAGCGTC14100.021.5583579
GCCGTAT6500.021.18702958-59
GGTCGCC7600.020.9313854-55
CAGTACG7750.020.53396834-35
CCGTATC7300.019.19075460-61
AGTACGT8050.019.17745636-37
ATACGGC3557.8216544E-1118.7289415
GTACGTG8450.018.55071836-37
CGTATCA7950.018.21904660-61
TGGTCGC8250.018.13106354-55
TACGGCG1350.009414840517.589356
GTGGTCG8900.017.34073652-53
AGAGCGT17300.017.2952468
GATCGGA18450.016.9994321
TCGGAAG19350.016.9345683
ATCGGAA19200.016.3241272
CGTTGGA19950.015.7212781
Produced by FastQC (version 0.11.5)