FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5957067
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA4150.032.04368258-59
GTCGCCG5150.025.81965656-57
TCGCCGT5500.024.6083156-57
GCCGTAT6500.021.55465558-59
GGTCGCC6800.020.2509354-55
TGGTCGC6850.020.10311354-55
CCGTATC7050.019.2002360-61
CGTATCA7550.018.87230760-61
GTGGTCG7300.018.53895252-53
GAGCGTC15200.018.12269
GATCGGA17050.017.8284591
AGAGCGT16150.017.349778
ATCGGAA17550.016.2364852
TCTCGGT9800.014.78257348-49
TCGGAAG19350.014.7263623
AAGAGCG19450.014.6500317
CGGAAGA20800.013.6997634
CGTCGTG10050.013.47119412-13
GCGTCGT10100.013.16834410-11
AGCGTCG10350.012.85026610-11
Produced by FastQC (version 0.11.5)