FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5422617
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA20600.016.1705531
CGTATGC5250.014.92742546-47
TATGCCG4800.014.84226948-49
CTCGTAT4950.014.39283444-45
TCGTATG5450.013.94388344-45
AAACGGG25250.013.7350387
AACGGGA20100.013.2361118
TCTCGTA5200.012.78747842-43
GAAACGG26700.011.9215296
ACGGGAT14700.011.6346579
GGAAACG26350.011.538355
ATGCCGT6050.011.38316248-49
AACGGGT5555.5811324E-711.1280438
GATCGGA13450.010.9681521
ATCGGAA14350.010.9382412
TCGGAAG14950.010.8012493
TGCCGTC6800.010.47718550-51
GCCGTCT6850.010.05401950-51
CGGAAGA16350.09.2954084
GATTTCG11807.2759576E-129.2595575
Produced by FastQC (version 0.11.5)