FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7062505
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT162570.23018744765490431TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA39050.045.8281441
ATCGGAA40200.044.730132
TCGGAAG40500.044.2104873
CGGAAGA41650.043.219074
TATGCCG21850.039.99638448-49
CGTATGC23350.037.73293746-47
TCTCGTA22600.037.19849442-43
CTCGTAT23400.037.0436444-45
TCGTATG24100.036.85455744-45
ATGCCGT25050.034.9818748-49
TGCCGTC23050.033.5874650-51
GCCGTCT23050.032.14505450-51
ATCTCGT26450.031.42482842-43
CGTCTTC24250.028.00817552-53
CCGTCTT24750.027.53830352-53
AATCTCG31550.026.79574240-41
AGAGCAC69200.026.3627198
ACACGTC34800.026.2039712-13
CGTCTGA34350.025.99363516-17
GTATGCC34700.025.86996846-47
Produced by FastQC (version 0.11.5)