FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3626521
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA3250.032.8761258-59
TCGCCGT3500.029.84922456-57
GTCGCCG3950.027.6508956-57
CGGCATA2301.3460522E-1024.7816738
TGGTCGC4450.024.00949154-55
GTGGTCG4850.022.51964252-53
GAGCGTC9100.022.4457559
GCCGTAT4700.022.228358-59
CCGTATC5050.022.09855360-61
GGTCGCC4850.022.02932754-55
AGAGCGT10400.021.4655368
CGTATCA5400.019.78682760-61
GATCGGA11800.018.4066871
CCGTGTA6100.018.29571538-39
CGTTGGA12200.018.1988161
TCTCGGT6600.017.6303348-49
ATCGGAA11350.017.5769142
GCGTCGT5950.017.56345210-11
AAGAGCG12850.017.3721667
TCGGAAG11800.017.308673
Produced by FastQC (version 0.11.5)