FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6271851
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7500.024.69736748-49
GATCGGA15650.023.1118981
CGTATGC8000.022.55964546-47
TCGTATG8450.022.48307844-45
ATCGGAA17150.021.9091382
CTCGTAT8350.021.89912444-45
TATCTCG8300.020.5997240-41
TCTCGTA8600.019.88160142-43
TCGGAAG18650.019.8624863
ATGCCGT9650.019.19484348-49
CGGAAGA20500.017.6072464
TGCCGTC11050.015.68881150-51
CGTTGGA24650.015.4457651
AACGGGA23300.015.29252058
ATCTCGT10900.015.25066842-43
ACGGGAT18750.014.1892829
AAACGGG28200.013.9828527
GCCGTCT11750.013.54148850-51
GAAACGG29500.013.2056146
CGTCTGA14350.012.90746516-17
Produced by FastQC (version 0.11.5)