FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4611408
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT55710.1208090891111782Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA7000.042.06062758-59
GAGCGTC15050.038.5021979
TCGCCGT7800.037.74569356-57
GTCGCCG8500.035.75455556-57
GATCGGA17550.034.365471
GCCGTAT8750.033.9198658-59
AGAGCGT17100.033.607228
ATCGGAA19000.032.493422
GGTCGCC9750.032.143554-55
TCGGAAG19500.031.4173953
TGGTCGC9700.031.33012254-55
CGTATCA9600.030.42286560-61
GTGGTCG10150.030.40926252-53
CGGAAGA21100.029.9359934
AAGAGCG20150.028.989487
CCGTATC10250.028.95692360-61
CGTCGTG11150.025.77628912-13
GCGTCGT11150.025.7748910-11
TCTCGGT12550.025.54679148-49
TCGGTGG12800.024.49001550-51
Produced by FastQC (version 0.11.5)